![]() El estudio se diseñó para examinar los cambios morfológicos de la ultraestructura mitocondrial en la corteza cerebral con la actividad de la glutatión peroxidasa (GPX) y la producción de radicales libres (RL) en el tejido cerebral durante la exposición a la hiperoxia. Se sabe que la exposición a la hiperoxia normobárica (HN) aumenta la producción de especies reactivas de oxígeno (ERO) por parte de las mitocondrias. ![]() KEY WORDS: Cortical Brain Morphological pathological alterations Normobaric hyperoxia Reactive oxygen species Reactive nitrogen species Ultrastructure Normobaric-hyperoxia inducts mitochondria oxidative stress (MOS) and the subsequent rise of ROS causes variety of ultrastructure morphological pathological alterations in the organelles of cortical brain cells. These mitochondria pathological alterations were associated with damages of myelin, axonal and cellular organelles. Ultrastructure examination showed that O2 breathing for 48 h resulted in giant and swelled mitochondria associated with diluted inner membrane and damaged cristae. Pair-wise means comparisons showed that NH elevated the average (+SE) GPX activity significantly (p<0.05) from the baseline control value of 5670.99+556.34 to13748.42+283.4.19+1529.26 U/L with increasing length of NH exposure period from 24 to 48 h, respectively. Analysis of variance (ANOVA) showed that the main effects of O2 exposure periods were significant (p<0.05) for GPX and FR. Following the exposure periods, animals were sacrificed and cortical tissues were divided randomly into two parts the first part was processed for the ultrastructural examination and the second was homogenized for GPX and FR determinations. The experimental groups were exposed to NH for 24 and 48 h continuously. The present study was designed to examine mitochondrial ultrastructure morphological changes in the cortical brainin relation to glutathione peroxidase (GPX) activity and free radicals (FR) productions in brain tissue during hyperoxia exposure. Exposure to normobaric hyperoxia (NH) is known to increase the production of reactive oxygen species (ROS) by mitochondria.
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